双氢青蒿素对脂多糖诱导的断奶仔猪肝氧化应激的影响

doi:10.11843/j.issn.0366-6964.2019.10.021

Abstract

Abstract: In order to study the effects of dihydroartemisinin (DHA) on oxidative stress in weaned piglets, a model of oxidative stress was established by injection of lipopolysaccharide (LPS). Thirty healthy weaned piglets were randomly divided into control group (CON), model group (LPS) and treatment group (LD, LPS + DHA). Each group had 10 replicates. CON group and LPS group were fed with basal diet, LD group was fed with basal diet + 80 mg·kg^-1 DHA. Each group was fed with corresponding diet for 21 days. LPS group and LD group were intraperitoneally injected with 100 μg·kg^-1 LPS at 4 hours before slaughter. CON group was injected with the same dose of sterile saline. Compared with the CON group, the contents of malondialdehyde (MDA), protein carbonyl (PC), 8-hydroxy-2-deoxyguanosine (8-OHdG) and hydrogen peroxide (H₂O₂) in the liver of LPS group were significantly increased (P<0.05). Meanwhile, the activities of total antioxidant capacity (T-AOC), catalase (CAT), glutathione peroxidase (GPx) and the contents of reduced glutathione (GSH) in the liver of LPS group were significantly decreased (P<0.05). Compared with LPS group, the contents of MDA, PC, 8-OHdG and H₂O₂ in LD group decreased significantly (P<0.05), and the activities of CAT, T-AOC and GPx increased significantly (P<0.05). In addition, the expression of nuclear factor E2-related factor 2 (Nrf2) and CAT and the protein expression of Nrf2 and HO-1 in liver of LPS piglets were significantly lower than those of CON group (P<0.05). DHA treatment significantly reversed the above changes induced by LPS (P<0.05). The results showed that DHA could alleviate oxidative stress in weaned piglets induced by LPS by regulating Nrf2/ARE signaling pathway, which provided a theoretical basis for reducing oxidative stress in livestock production.

CLC Number:

ZHAO Yongwei, NIU Yu, HE Jintian, JI Shuli, ZHANG Lili, WANG Chao, WANG Tian. Effects of Dihydroartemisinin on Oxidative Stress in Liver of Weaned Piglets Induced by Lipopolysaccharide[J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2019, 50(10): 2139-2146.

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Effects of Dihydroartemisinin on Oxidative Stress in Liver of Weaned Piglets Induced by Lipopolysaccharide

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